NSTL国家科技图书文献中心

1. Isolation and Characterization of Extracellular Vesicles from Natural Killer Cells NSTL国家科技图书文献中心

Sara G. Dosil | Francisco Sánchez-Ma...... - 《Current protocols.》 - 2025,5(4) - e70125～e70125 - 共14页

摘要：Natural killer (NK) cells are critical | components of the immune system, recognized for their | ability to identify and eliminate target cells | undergoing various forms of stress. Beyond their direct | cytotoxic activity, NK cells secrete extracellular

关键词： EVs; NK cells; exosomes; extracellular vesicles; lymphocytes; natural killer cells; peripheral

2. High-sensitivity Epimutations Testing with MS-HRM NSTL国家科技图书文献中心

Taryma-Lesniak, Olga | Sokolowska, Katarzyn...... - 《Current protocols.》 - 2025,5(2) - e70094～e70094 - 共18页

摘要：Epimutation is defined as any heritable change | in gene activity that does not affect the actual | sequence of DNA. One of the most researched epimutations | is promoter methylation of breast cancer | susceptibility gene BRCA1. This epimutation may arise de novo

关键词： BRCA1 | DNA methylation | epimutation | MS-HRM

3. Visualization of Efavirenz-Induced Lipid Alterations in the Mouse Brain Using MALDI Mass Spectrometry Imaging NSTL国家科技图书文献中心

Phulara, Nav Raj | Seneviratne, Herana ... - 《Current protocols.》 - 2025,5(2) - e70108～e70108 - 共17页

摘要：This article highlights experimental | procedures and troubleshooting tips for the utilization of | matrix-assisted laser desorption/ionization mass | spectrometry imaging (MALDI MSI) methods for detecting and | visualizing lipid alterations in the mouse brain tissue in

关键词： brain | drug response | efavirenz | lipids | MALDI mass spectrometry imaging

4. Verifying Proper Function of the Aerosol Evacuation System Prior to Sorting Potentially Infectious Samples NSTL国家科技图书文献中心

Reifel, Kristen M. | Aspland, Avrill ... - 《Current protocols.》 - 2025,5(3) - e70123～e70123 - 共24页

摘要：High concentrations of aerosols can be | generated within the sort collection area of cell sorters | during instrument failures that cause the stream to | deviate, such as a partial nozzle obstruction. Complete | containment of these aerosol particles becomes essential for

关键词： aerosol containment | biosafety | cell sorting | flow cytometry | risk assessment | risk mitigation

5. A Physiologically Relevant In Vitro Model of Nonreplicating Persistent Mycobacterium tuberculosis in Caseum NSTL国家科技图书文献中心

Xie, Min | Osiecki, Paulina ... - 《Current protocols.》 - 2025,5(3) - e70118～e70118 - 共19页

摘要：Tuberculosis (TB) remains one of the leading | infectious causes of death worldwide. Persistent bacterial | populations in specific microenvironments within the host | hamper efficient TB chemotherapy. Caseum in the | necrotic core of closed granulomas and cavities of

关键词： caseum | drug tolerance | granuloma | Mycobacterium tuberculosis | nonreplicating persistence

6. Protocol for Controlling the Strand Selectivity of siRNA Using Acyclic Artificial Nucleic Acids NSTL国家科技图书文献中心

Ariyoshi, Jumpei | Asanuma, Hiroyuki ... - 《Current protocols.》 - 2025,5(3) - e70103～e70103 - 共24页

摘要：Small interfering RNA (siRNA) has emerged as a | promising therapeutic candidate against previously | intractable diseases. An effective siRNA must have high on | -target activity while off-target effects are minimized | . This balance can be achieved by enhancing the

关键词： small interfering RNA | strand selectivity | off‐target | luciferase assay | RNA‐induced silencing complex | off-target | RNA-induced silencing complex

7. Isolation and Characterization of Human Tissue Resident Memory T cells NSTL国家科技图书文献中心

Jensen, Isaac J. | Wells, Steven B.... - 《Current protocols.》 - 2025,5(3) - e70120～e70120 - 共25页

摘要：The majority of immune cells in the human body | exist within the tissues rather than in the | circulation. Nevertheless, most of our knowledge of the | human immune system is biased towards the | characterization and understanding of circulating immune cell

关键词： human tissue | immune cells | single cell suspension | T cells | tissue resident

8. Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods NSTL国家科技图书文献中心

Beacher, Nicholas J. | Wang, Michael W.... - 《Current protocols.》 - 2025,5(1) - e70090～e70090 - 共21页

摘要：In vivo calcium imaging in freely moving rats | using miniscopes provides valuable information about | the neural mechanisms of behavior in real time. A | gradient index (GRIN) lens can be implanted in deep brain | structures to relay activity from single neurons. While

关键词： custom | imaging | miniscope | nucleus accumbens | surgery

9. Genetically Engineered and Implantable Mouse Brain Tumor Models: Characterization by Immunohistochemistry and Flow Cytometry NSTL国家科技图书文献中心

Mirji, Apoorva | Singh, Gurveer ... - 《Current protocols.》 - 2025,5(1) - e70080～e70080 - 共28页

摘要：Gliomas are aggressive tumors with a poor | prognosis. The protocols presented here outline the | methods used to study tumor progression, the tumor | microenvironment (TME), and the effects of experimental | treatments. The Sleeping Beauty (SB) transposase system

关键词： flow cytometry | genetically engineered mouse models | Gliomas | immune microenvironment | immunohistochemistry | preclinical model

10. Simple Isolation of Human Bone Marrow Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells NSTL国家科技图书文献中心

Tonyali, Guelsena | Kilic, Emine ... - 《Current protocols.》 - 2025,5(1) - e70081～e70081 - 共22页

摘要：Bone marrow adipose tissue (BMAT) has garnered | significant attention due to its critical roles in leukemia | pathogenesis, cancer metastasis, and bone marrow failure | . BMAT is a metabolically active, distinct tissue that | differs from other fat depots. Marrow adipocytes

关键词： adipose tissue culture | bone marrow adipose tissue | marrow adipose tissue characterization | marrow adipose tissue isolation | mesenchymal stem/stromal cell