Wessels, Hans-Herman... |  Mendez-Mancilla, Ale...... -  《Nature methods》 - 2023,20(1) - 86～+ - 共26页

摘要：Pooled CRISPR screens coupled with single-cell RNA-sequencing have enabled systematic interrogation of gene function and regulatory networks. Here, we introduce Cas13 RNA Perturb-seq (CaRPool-seq), wh...

关键词： CIRCUITS |  SCREENS

Jiang, Longda |  Dalgarno, Carol... -  《Nature cell biology》 - 2025,27(3) - 505～517 - 共33页

摘要：Recent advancements in functional genomics have provided an unprecedented ability to measure diverse molecular modalities, but predicting causal regulatory relationships from observational data remain...

Kaufmann, Kerstin B. |  Zeng, Andy G. X.... -  《Nature immunology》 - 2021,22(6) - 723～+ - 共32页

摘要：Continuous supply of immune cells throughout life relies on the delicate balance in the hematopoietic stem cell (HSC) pool between long-term maintenance and meeting the demands of both normal blood pr...

Hao, Yuhan |  Hao, Stephanie... -  《Cell》 - 2021,184(13) - 3573～+ - 共44页

摘要：The simultaneous measurement of multiple modalities represents an exciting frontier for single-cell genomics and necessitates computational methods that can define cellular states based on multimodal ...

Stuart, Tim |  Butler, Andrew... -  《Cell》 - 2019,177(7) - 1888～+ - 共36页

摘要：Single-cell transcriptomics has transformed our ability to characterize cell states, but deep biological understanding requires more than a taxonomic listing of clusters. As new methods arise to measu...

Mimitou, Eleni P. |  Cheng, Anthony... -  《Nature methods》 - 2019,16(5) - 409～+ - 共6页

摘要：Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-comp...

Butler, Andrew |  Hoffman, Paul... -  《Nature biotechnology》 - 2018,36(5) - 411～+ - 共15页

摘要：Computational single-cell RNA-seq (scRNA-seq) methods have been successfully applied to experiments representing a single condition, technology, or species to discover and define cellular phenotypes. ...

Upadhaya, Samik |  Sawai, Catherine M.... -  《The Journal of Experimental Medicine》 - 2018,215(11) - 2815～2832 - 共18页

摘要：Adult hematopoiesis has been studied in terms of progenitor differentiation potentials, whereas its kinetics in vivo is poorly understood. We combined inducible lineage tracing of endogenous adult hem...

Kleppe, Maria |  Spitzer, Matthew H.... -  《Cell stem cell》 - 2018,22(2) - 277～277 - 共1页

Kleppe, Maria |  Koche, Richard... -  《Cancer Cell》 - 2018,33(4) - 785～787 - 共4页